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Expression of WT FLAG-rOXCT1 or FLAG-rOXCT1 K421R was reconstituted in endogenous OXCT1-depleted Huh7 cells. Cells were treated with or without IGF for 6 h. FLAG-tagged proteins were immunoprecipitated using an anti-FLAG antibody. The activities of OXCT1 were analyzed using thein vitroOXCT1 activity assay. The data represent the average of three independent experiments (mean ± SD).*p< 0.01;***p< 0.001; ns, not significant.
#Bar Plot#Error Bars#Clustered#FLAG-rOXCT1 Proteins#Huh7 Cells#IGF#Immunoprecipitation#Activity Assay
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