Line Plot from Scientific Research

(TOP) Schema of retroviral vectors expressing CAR and NGFR-P2A-GLUT1. NGFR is used as a selectable membrane marker of GLUT1-OE cells. (BOTTOM) Representative flow cytometry histogram of GLUT1 surface expression using a GLUT1-specific H2RBD-GFP ligand, in control CD19 and HA CAR T cells +- NGFR-GLUT1 vector (CD19-GLUT1, HA-GLUT1). Analysis of GLUT1OE CAR T cells performed by gating on CAR+/NGFR+ populations (percentage of double-positive >80% for each experiment unless otherwise noted). FMO Fluorescence minus one control.

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