Gel from Scientific Research

Open access visualization of Gel, In Vitro Kinase Assays, GST-ERK2, His-SUCLA2, His-SUCLA2 Mutants
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DOI: 10.1016/j.molcel.2024.12.025

DOI

In vitro kinase assays were carried out by mixing purified active GST-ERK2 with WT His-SUCLA2 or His-SUCLA2 S76A, S124A, S199A, and T341A mutants in the presence of [-32P]-ATP. The reaction mixture was separated for autoradiography and immunoblotting analyses.

#Gel#In Vitro Kinase Assays#GST-ERK2#His-SUCLA2#His-SUCLA2 Mutants#ATP#Autoradiography#Immunoblotting Analyses

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