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Closer views of mean lifetime changes at spines during the first 8 uncaging pulses in wild-type 1dV(WT) ( n = 12 spines, 5 neurons), 2dV(WT) ( n = 31 spines, 10 neurons), 2dV(T286A) ( n = 28 spines, 3 neurons) and 2dV(T305D/T306D) ( n = 39 spines, 5 neurons).
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