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Summary of the peak (6th–11th uncaging pulses) of fluorescence lifetime changes in stimulated spines. 2dV ( n = 31 spines, 10 neurons) showed higher sensitivity than 1dV ( n = 12 spines, 5 neurons). Two-tailed unpaired t -test, ( t 41 = 4.26, P = 1.18 × 10 –4 ).
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