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MDA-MB-231 cells were immunoprecipitated with an anti-SNRNP200 antibody, with IgG serving as a negative control. The IP data were analyzed by western blot analysis (on the left). qPCR analysis of the U4, U5, and U6 snRNA levels in the input, RNase A − , and RNase A + groups. The relative U4, U5, and U6 snRNA levels were normalized to that of the input using the 2 −Ct method (on the right). qPCR analysis data are presented as the mean ± SEM.

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