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Cleavage activity of WT KmAgo and 27 Km-APs at 37 C on ssDNA. Proteins are loaded with ssDNA guide and then incubated with ssDNA target in a 2:1:1 molar ratio (protein:guide:target). These activities are defined by the slope of the fluorescence intensity vs time curve 44 . Results from two independent experiments were quantified, with the associated standard deviations represented by error bars.
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