Bar Plot from Scientific Research

BRET2 assay to evaluate PrRP20-induced dissociation of heterotrimeric Gq protein (ΔpEC50 = pEC50 of PrRP20 to a specific PrRPR variant – pEC50 of PrRP20 to WT PrRPR; yellow column means ΔpEC50 ≤ –1; blue column means ΔpEC50 ≤ –2; red column means ΔpEC50 ≤ –3). Data are presented as means ± SEM; n = 3 independent samples, each consisting of triplicate measurements. Significance was determined by one-way ANOVA with Dunnett’s multiple comparisons test. NA, no activity; ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001. Exact P values are provided in Supplementary Table S2.

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