Microscopy from Scientific Research

G3BP1::RNA co-condensates were formed using 10 M GFP-G3BP1 (green), 200 ng/L total hRNA, and 50 ng/L cy5-labeledluciferaseRNA (magenta) and hardened for 1 h. Co-condensates were then treated with equal volumes enzyme buffer (STAR Methods), PK (0.1 U/L), RNase A (1.43 g/L), or TURBO DNase (0.28 U/L) for 15 min before imaging. The pretreated sample (middle) was made by incubating 10 M GFP-G3BP1 with 0.1 U/L PK for 15 min before adding to the condensate reaction. All scale bars are 1 m.

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