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Experimental scheme for scRNA-seq analysis of QSCs freshly FACS-isolated from uninjured TA muscles of 8-week-old Pax7 CreERT2/+ ; Rosa26 mTmG/+ mice, or ASCs FACS-isolated from injured TA muscles on day 1.5 after CTX injection. Each biological replicate of scRNA-seq comprises a pool of SCs from five mice (for QSCs or ASCs).
#Workflow#Flowchart#Experimental Scheme#Single-Cell RNA Sequencing#Quiescent Satellite Cells#Tibialis Anterior Muscle#Pax7 CreERT2/+; Rosa26 mTmG/+ Mice#Activated Satellite Cells#Cardiotoxin Injection
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