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Transplantation workflow showing the cryopreservation of CD34 + hematopoietic cells from the cell suspension along with MACS-isolated CD34 + cells from the EB. MACS-enriched EB cells were not collected for all experiments. Cryopreserved cells were thawed and transplanted immediately into NBSGW immune-deficient mice by tail-vein injection. Peripheral blood was analyzed at 12 weeks to screen for engraftment and hematopoietic tissues were analyzed for human cells at time points up to 24 weeks (Supplementary Tables 2 and 3).

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