TTP-WT and TTP-TZFmut localization in HEK-293 cells treated with dox (1 μg/mL, 3.5 h) to induce Myc-tagged construct expression (Myc antibody, red) or with vehicle (DMSO). Nucleus: DAPI (blue). Profile lines were drawn through representative cells (enlarged 2-fold in insets, scale bar, 25 μm) for quantitation of mean fluorescence intensity (MFI) shown in (D). TTP-TZFmut and DAPI profiles were similar, implying nuclear localization of the protein.
