Gel from Scientific Research

Open access visualization of Gel, Doxycycline, WT-APX, TZFmut-APX, GFP-APX
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Doxycycline-induced (1 ug/mL dox, 3.5 h) expression of WT-APX, TZFmut-APX, and GFP-APX in HEK-293 cells analyzed by western blotting. Anisomycin (+aniso, 100 uM, 1.5 h) was used as indicated. Immunoblot (IB): APX and vinculin (loading control). TTP-APX and GFP-APX fusion proteins are indicated. Slower-migrating TTP-APX fusions in anisomycin samples correspond to hyperphosphorylated proteins.

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