R H of HSA as obtained by continuous scan (orange points) and step scan (blue points) measurements. Data points (mean) were obtained from at least triplicate measurements at the respective sample concentration (see source data for number of repeats). Error bars denote standard deviations. The dashed line indicates the average literature value (mean) for HSA ( R H = 3.73 0.40 nm) 67 , 68 , 69 , 70 with the green band depicting the standard deviation of literature values. Source data are provided as a Source Data file.