Line Plot from Scientific Research

A complementary standard full-frame FLIM measurement with 530 nm excitation and detection of donor emission. Shown is the donor emission decay in the presence of acceptor (red) and with acceptor bleached (black). The curves are overlaid with fits (dashed) as described in the SI, fit residuals are shown as well (dotted). The number of experimental repetitions is described in the Statistics and Reproducibility section in Methods.

DOI