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Binding curves of calstabin-2 to WT RyR2. Concentration of calstabin-2 is 5, 10, 50, 100, 500, and 1000 nM (from black to magenta), and RyR2 is 5 nM. Reactions were performed as duplicates. Data are presented as mean values and error bars as SD. All points were simultaneously fitted using the equation Association Kinetics - Two or more concentrations of ligand in GraphPad. Fitting curves are shown in gray, R 2 = 0.9895.
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