Illustration from Scientific Research

Schematic representation of 3D-CIS. Arrays of Lac and Tet operons are inserted next to the transgenic and endogenous Fab-7 elements. Expression of a TetR-LacI fusion protein binding to both arrays promotes contacts between the two loci. Illustrative micrographs represent nuclei from embryos with 3D-CIS in the OFF or ON state, with FISH highlighting the regions surrounding the Fab-7 elements. Nuclei are stained with DAPI in blue, the 37B and 89E loci are stained in red and green, respectively. Scale bars represent 1 μm. See also Figures S8 and S9.

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