Quantification of intracisternally injected Y39-1 MPI (in arbitrary units, a.u.) and of the fluorescent area (expressed as % of section area), showed that exposure to 40 Hz light flickering increased the glymphatic influx in WT mice, but not in ENT2-KO mice; analysis was performed in six sections per animal ( n = 5–7 mice/group, mean ± SEM in the bar graphs, * P < 0.05, *** P < 0.001, ns, not significant, one-way ANOVA with Tukey’s multiple comparison test).