Gel from Scientific Research

Open access visualization of Gel, 35S-methionine-labeled CCT4 Substrates, E1, E2 (UBE2A), WT FLAG-Ub
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DOI: 10.1016/j.molcel.2025.01.002

DOI

35S-methionine-labeled CCT4 substrates produced as outlined in (A) were incubated with E1, E2 (UBE2A), WT FLAG-Ub, and recombinant UBR4 and KCMF1. The samples were then analyzed by autoradiography, either directly (total products) or after a denaturing FLAG-Ub PD.

#Gel#35S-methionine-labeled CCT4 Substrates#E1#E2 (UBE2A)#WT FLAG-Ub#Recombinant UBR4#KCMF1#Autoradiography#FLAG-Ub PD

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