Box plots showing a significant difference (two-sided Mann–Whitney U -test) between the median localizations per cluster ( i ) and median cluster area ( j ) in somatic ( n = 22) and hiPSC ( n = 21) nucleoli. All the box plots depict the median (horizontal line inside box), 25th and 75th percentiles (box), and 25th or 75th percentiles 1.5 interquartile range (whiskers). Distributions were compared as indicated, using the Mann–Whitney U -test. ** P < 0.01.