ChIP PCR analysis of H3K4me3 levels at the promoter regions of Nanog and Zic2 and at distal enhancer region of Pou5f1 in the absence and presence of YAP inhibitor. Data were normalized by the total H3 value in each group. Positive control (TCP, 2iL) group was set as 1 ( Nanog promoter: n = 4 biologically independent experiments; Pou5f1 distal enhancer and Zic2 promoter: n = 3 biologically independent experiments; data are presented as means s.d.; statistical significance was calculated via a one-way ANOVA with Bonferroni’s multiple comparisons test for groups without YAP inhibition and via a two-tailed Student’s t -test for comparing inhibition and the corresponding untreated groups).
