Microscopy from Scientific Research

Representative BMS topographical map and PSC image. Cells were stained on day 3 with Hoechst 33342 to visualize the nuclei of the whole population (blue), whereas the NANOG hi (green) and STELLA hi (white) cells were filtered out to show the naive population. Bright-field (BF) image of BMS and nuclei staining of PSCs (i); naive PSCs overlaid with BMS motif map (ii): concave (red), convex (cyan), flat (grey), BSCR+ (yellow) and BSCR (black). Naive PSC distribution in BSCR+ and BSCR areas in the concave region (iii). Scale bars, 100 m.

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