Representative bright-field and fluorescence images at timing of EGA in mouse 2-cell and bovine 8-cell embryos after microinjection with indicated plasmids.n, number of embryos with fluorescence signal over total number of embryos microinjected in two independent experiments (N= 2). Fluorescence intensity is heterogeneous between blastomeres in bovine embryos, reflecting known asynchrony of cells entering EGA in cow. See alsoFigure S7.
