Effect of the sequence feature ( x axis) on readthrough efficiency ( y axis) in HEK293T_LP cells, colored by the drug. The top and bottom sides of the box are the upper and lower quartiles, respectively. The box covers the interquartile interval, where 50% of the data are found. The horizontal line that splits the box in two is the median. Finally, the effect of amino acids encoded by A-ending codons on readthrough efficiency across drugs, where codons ended in A display higher readthrough compared to the rest of the codons ( n = 7,989, adjusted P < 6 x 10 -5 for DAP, G418 and SRI, one-sided Wilcoxon signed-rank test). The nucleotide upstream of the PTC is colored.
