Knockout of TREX1 in activated T-cells increases CRISPRCas9-mediated HDR from unprotected ssODN templates ( n = 5, P = 2.8 x 10 -5 , P = 0.3815 for CXCR4 and P = 6.1 x 10 -8 , P = 0.1103 for FANCD2 , n = 2 for UROS ). Black dots represent measurements with unprotected (un) ssODN templates, and blue dots represent measurements with 5 and 3 protected (PT) ssODN templates. Each dot represents an individual biological replicate, and bars represent the mean. All P values were calculated using an unpaired and two-sided t-test: < 0.05, ** P < 0.01, *** P < 0.001. Source data are provided as a Source Data file. NT, non-targeting; IB, immunoblotting; KO, knockout; NS, not significant.
