Bar Plot from Scientific Research

CRISPRi knockdown of TREX1 significantly increases CRISPRCas9-mediated HDR in FANCD2 deficient LCLs. Both cell backgrounds were stably transduced with up to three different sgRNAs targeting TREX1 , and a BFP-to-GFP assay was used to measure HDR efficiency. In Fig. 1a,c,d , each dot represents an individual biological replicate, and bars represent the mean ( n = 4 for sgNT in c and sgTREX1-3 in d , n = 3 for the rest of the samples in c and d , biologically independent experiments, P = 0.031, 0.0065 and 0.0088 for c and P = 0.0234, 0.0096 for d , respectively). All P values were calculated using an unpaired and two-sided t -test, * P < 0.05, ** P < 0.01, *** P < 0.001. NT, non-targeting. Source data are provided as a Source Data file.

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