The FOV averages of the SA-647/GFP fluorescence ratio per cell for the conditions shown in f (n = 12 FOVs per condition; KCl versus DA: P = 0.547; KCl versus DOI: P = 0.008; KCl versus KCl: P = 6.5 × 10 −4 , Tukey’s post hoc multiple-comparison’s test following a one-way ANOVA, F 3,44 = 7.373, P = 4.2 × 10 −4 ). All scale bars, 300 µm. * P < 0.05, * *P < 0.01, *** P < 0.001, **** P < 0.0001.
