The FOV averages of the SA-647/GFP fluorescence ratio per cell for the data shown in a (n = 6 FOVs per condition; biotin KCl versus +biotin +KCl: P = 1.8 × 10 −12 ; biotin +KCl versus +biotin +KCl: P = 3.0 × 10 −11 ; +biotin −KCl versus +biotin +KCl: P = 1.4 × 10 −10 ; biotin KCl versus +biotin −KCl: P = 0.013; Sidak’s post hoc multiple-comparison’s test following a two-way ANOVA, F 1,20 = 59.43, P = 2.1 × 10 −7 ).
