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For FLiCRE, the FOV average of the UAS::mCherry cell fluorescence was calculated following a variable delay period after light Ca 2+ stimulation ( n = 11 FOVs for conditions with 0 h delay; n = 12 FOVs for conditions with 2, 4, 6 and 8 h delay; 6 h: P = 4.6 x 10 -5 ; 8 h: P = 2.4 x 10 -28 ; Sidak’s post hoc multiple-comparison’s test following a two-way ANOVA, F 4,108 = 46.46, P = 1.2 x 10 -22 ).
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