The mean FOV averages of the SA-647/GFP fluorescence ratio per cell for the different stimulation times shown in e ( n = 10 FOVs per condition). The untreated condition is shown on the left. Data are plotted as the mean ± s.e.m. All scale bars, 300 µm. *** P < 0.001, **** P < 0.0001. NS, not significant.