The FOV averages of the SA-647/GFP fluorescence ratio per cell for the three conditions shown in a ( n = 8 FOVs per condition; biotin − Ca 2+ versus Ca 2+ /wash/biotin: P = 0.75; biotin − Ca 2+ versus biotin + Ca 2+ : P = 4.9 × 10 −8 ; Ca 2+ /wash/biotin versus biotin + Ca 2+ : P = 1.3 × 10 −8 ; Tukey’s post hoc multiple-comparison’s test following a one-way analysis of variance (ANOVA), F 2,21 = 56.37, P = 3.6 × 10 −9 ).
