Measurement of the FC of PAX6 and NR2F1 (CoupTF1) gene expression using RTqPCR following bath application of BMP, with two biological replicates and two technical replicates each. One-way ANOVA using Tukey’s multiple-comparisons test was used to calculate adjusted P values. For PAX6, control versus 10 ng ml –1 : mean diff. 0.50 with 95% confidence interval (CI) (0.40, 0.61), adjusted P < 1 × 10 −4 . For PAX6, control versus 50 ng ml –1 : mean diff. 0.57 with 95% CI (0.47, 0.68), adjusted P < 1 × 10 −4 . For NR2F1, control versus 10 ng ml –1 : mean diff. −0.91 with 95% CI (−1.88, 0.06), adjusted P = 0.066. For NR2F1 control versus 50 ng ml –1 : mean diff. −1.43 with 95% CI (−2.40, −0.45), adjusted P = 0.0068. In g , *adjusted P ≤ 0.05, **adjusted P ≤ 0.01, ***adjusted P ≤ 0.001, ****adjusted P ≤ 0.0001. Error bars represent s.d.
