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HeLa cells expressing ProDOL and LynG probes were stained with varying concentrations of SNAP-SiR (1, 2) and Halo-TMR (3, 4) for 30 min prefixation (green) or postfixation (orange). Determined DOL using ProDOL at varying substrate concentrations (1 and 3). Unspecific labeling density measured in HeLa cells expressing LynG at varying substrate concentrations (2 and 4). Data are median s.d. (crosses, shaded bands) for 6-20 cells per condition.
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