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Open access visualization of Illustration, Schematic Model, DOL, Single-Molecule Colocalization Analysis, eGFP Reference
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A schematic model to determine the DOL based on single-molecule colocalization analysis between an eGFP reference and tag-target label. Within the ProDOL analysis, four labeling types can be discerned (specific label, specific label with no reference, nonlabeled and unspecific label) and used to calculate the DOL and unspecific labeling density.

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