Aginome-XMU predictions for CD4+ T cells (y axis) for unconstrained admixtures including the level of CD4+ T cells indicated (x axis). Limit of detection (LoD) is the least frequency at and above which all admixtures are above background (i.e., statistically different from the baseline admixture of 0% spike in based on a raw / uncorrected, two-sided Wilcoxon p value), which is 6% in this case. Boxplots display median (center line), 25th and 75th percentiles (hinges), and 1.5x interquartile range (whiskers) over n = 10 in silico unconstrained spike-in admixtures. Limits of detection for indicated methods (rows) and cell types (columns) calculated using n = 10 in silico unconstrained spike-in admixtures in each of the ( B ) coarse- and ( C ) fine-grained sub-Challenges. Best/lowest LoD for each cell type highlighted in bold italics. Methods ordered according to their mean LoD. Comparator methods in bold. Source data are provided as a Source Data file.
