Pulldown assays of 12mer HS and FGF:FGFR complexes. Top panel show representative gels for FGFs 1,2,7 and 10 complexed with rFGFR1b, bottom panel shows the same FGFs with rFGFR2b. Lane 1: beads only, lane 2: non-3- O -HS, lane 3- 3ST1-HS, lane 4: 3ST3-HS. The molecular weight markers in kilodaltons (97 and 64 kDa) are indicated on each gel. Full gels are provided in the source data file. Graphs show quantitation of the FGFR complex separated by SDS-PAGE and silver-stained from independent samples. n = 3 for FGF1:FGFR1b, FGF1:FGFR2b, FGF7:FGFR1b, FGF7:FGFR2b; n = 4 for FGF2:FGFR1b, FGF10:FGFR1b, FGF10:FGFR2b; and n = 6 for FGF2:FGFR2b. Error bars: SM. One-way ANOVA with Dunnett’s test for multiple comparisons to non-3- O -HS, FGF2:FGFR1b: 3ST1-HS ** p = 0.0026, 3ST3-HS *** p < 0.0001; FGF10:FGFR1b: 3ST1-HS * p = 0.0136; FGF2:FGFR2b: 3ST1-HS * p = 0.0482; FGF10:FGFR2b: 3ST1-HS *** p = 0.0002 and 3ST3-HS *** p < 0.0001.
