Microscopy from Scientific Research

Immunostaining at E13 shows Hs3st3-dependent 3- O -sulfation (HS4C3V) in the basement membrane is reduced in DKO SMGs, whereas Hs3st1-dependent 3- O -sulfation (AT488 binding) is not affected. Representative single confocal images of HS4C3V (green), agrin (magenta), E-cadherin (cyan) and nuclei in blue. Right panel shows that HS4C3V staining in the basement membrane is dependent on heparan sulfate. The section was pretreated with heparinase III enzyme (HPT), which removes the 3- O -sulfated epitope recognized by the antibody. Scale bars, 5 µm. n = 3 WT and n = 4 DKO embryonic glands.

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