qPCR confirmed gene expression changes in female and male SMGs normalized to Rps29 and respective sex-matched WT (dotted line). WT-F and DKO-F: n = 3. WT-M and DKO-M: n = 4 or 7 SMGs. Unpaired two-tailed t -test compared to sex-matched WT (DKO-M: Krt14 ** p = 0.0045, Acta2 * p = 0.0188, Cnn1 ** p = 0.0091, Myh11 ** p = 0.0086, Mylk * p = 0.0157, Tagln * p = 0.0139, Postn * p = 0.0152, Krt19 * p = 0.0127, Muc13 * p = 0.0206, Klk1b24 * p = 0.0183, Klk1b21 * p = 0.0382, Egf * p = 0.0202, Ngf * p = 0.0107, Fgf1 * p = 0.0159, Fgf9 * p = 0.0167, Pdgfa * p = 0.0109, Tgfb2 * p = 0.0284, Mucl1 *** p < 0.0001, Lpo * p = 0.0338, Caecam1 ** p = 0.0058. DKO-F: Krt14 * p = 0.0232, Acta2 * p = 0.0186, Cnn1 ** p = 0.0010, Myh11 ** p = 0.0082, Mylk * p = 0.0321, Tagln ** p = 0.0026, Postn * p = 0.0418, Klk1b24 * p = 0.0421, Klk1b21 * p = 0.0210, Ctgf * p = 0.0191, Egf * p = 0.0354, Ngf * p = 0.0226, Pdgfa * p = 0.0112, Kitl * p = 0.0380, Aqp5 * p = 0.0163, Agt * p = 0.0433, Mucl1 *** p = 0.0004, Agr2 ** p = 0.0092).
