A TFSM can be used to visualize chlamydial inclusion via 4xExM. HeLa cells were infected with C. trachomatis at MOI 1 in the presence of TFSM 1 for 24 h, fixed and stained with AlexaFluor TM (AF)546-azide (headgroup) and BODIPY-FL-DBCO (backbone). Chlamydia was stained with an anti-chlamydial heat-shock protein 60 (HSP60) anti-body and an AF405 secondary antibody. Samples were 4x expanded and imaged with CLSM. n = 3. Scale bars: 8 µm (with 4-fold expansion factor ~ 2 µm).
