Identification of high metabolized TFSM levels in EBs by FLIM imaging of 4-fold expanded TFSM 1 -stained chlamydial inclusion labeled with BODIPY-FL-DBCO (left) as well as BODIPY-FL-DBCO and AF546-azide (middle, right). Zoomed image (right) of BODIPY-FL-DBCO and AF546-azide labeled TFSM shows higher fluorescence lifetimes of EBs in comparison to RBs, indicating a higher proportion of metabolized TFSMs. n = 1. Scale bars: 10 µm (with 4x expansion factor ~ 2.5 µm). Statistics: Two-sided unpaired Student’s t test ( d ). Bars represent means ± SD. n corresponds to biological replicates. Source data and detailed statistics are provided as a Source Data file.
