Microscopy from Scientific Research

Chlamydial inclusions possess lower FRET efficiency than host cell membranes. Samples were prepared as described in ( a ), and FRET efficiency was determined by acceptor bleaching. The acceptor fluorophore (AF546) was bleached in an area either containing a chlamydial inclusion (white line) or host cell membranes. Then, donor fluorescence before (pre) and after (post) bleaching was compared to determine FRET efficiency. In ( b ), samples incubated with TFSM 2 are depicted. Scale bars: 10 m. TFSM 1 : n = 5, TFSM 2 n = 4.

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