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Open access visualization of Microscopy, Fluorescence Lifetime Imaging Microscopy, FLIM, HeLa Cells, Confocal Imaging
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Overview fluorescence lifetime imaging microscopy (FLIM) images of infected, TFSM 1 -stained HeLa cells click-labeled with BODIPY-FL-DBCO (left) as well as BODIPY-FL-DBCO and AF546-azide (right) measured by confocal imaging at an irradiation intensity of 0.5 kW cm -2 . Chlamydia inclusions are marked with white lines. No intensity threshold was applied. n = 1. Scale bars, 20 m.

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