TFSMs are enriched within the Golgi and mitochondria. HeLa cells were treated with TFSM 1 for 2 h. Samples were fixed and clicked to BODIPY-FL-DBCO (backbone) or Atto647N-azide (headgroup). Golgi and mitochondria were visualized by an anti-Golgi matrix protein 130 (GM130) and anti-Peroxiredoxin (Prx3) antibody, respectively. n = 1. Scale bars: 25 m.