Chemical Structure from Scientific Research

Trifunctional sphingomyelin derivative TFSM 1 . The molecule can be coupled to different conjugation partners (e.g., biotin, fluorescent dyes, quencher molecules), offering a broad range of applications. Illustrated above, a FRET pair was coupled allowing to determine the cleavage status of the probe. The third functionality is the primary amino group for fixation in expansion microscopy, in this case, glutaraldehyde fixation. Figure 1a was created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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