Scatter Plot from Scientific Research

A NanoBiT-based DEP recruitment BRET assay was performed with FZD 7 -SmBiT, its respective cholesterol-binding site mutants, and β 2 AR-SmBiT as a control in HEK293A cells. For functional reconstitution of Nluc, membrane-anchored FLAG-LgBiT-CAAX was cotransfected. Data are presented in triplicates of three independent experiments with baseline-corrected BRET values based on conditions with 0% DEP-Venus. Data represent superimposed datapoints from three (FZD 7 wt and β 2 ) to four (FZD 7 mutants) independent experiments, each performed in triplicate. Error bars in both x and y directions represent SD

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