sam1Δ sam2Δ ( a ), met6Δ mht1Δ sam4Δ ( b ) and met6Δ mht1Δ sam4Δ cys4Δ ( c ) mutants (Y1504, Y1533 or 1534 & Y1660) were grown to exponential phase in SF-medium supplemented with 0.5 mM Cys, 0.1 mM Met and 0.1 mM SAM, and shifted to medium containing no sulfur. After 90 min, cells were replenished with 0.5 mM Cys, 0.1 mM Met or 0.1 mM SAM, as indicated. The schematics indicate: the sulfur component added after the 90-min starvation period (thick outline), the components that could be synthetized (thin outline), and components that could not be synthetized due to the mutation and therefore remained depleted (written in white on gray backgrounds). The Samples were collected before the shift (+), following the starvation period (–) and at the indicated time points following repletion. Transcript levels (graphs) were measured by RT-qPCR, and are relative to the 90-min total starvation time point. Fold induction is relative to t0. Data are mean ± SD of n = 3 independent experiments. Source data are provided with this paper.
