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GFP-Atg8 processing assay. The sam1 sam2 mutant expressing GFP-Atg8 from ATG8 endogenous promoter (Y1506) was grown and starved as above. Cells were collected at the indicated times. GFP-Atg8 expression and free GFP are relative to the 6-h time point in the no sulfur condition. Data are mean of two independent cultures.
#Bar Plot#Gel#GFP-Atg8 Processing Assay#sam1Δ sam2Δ Mutant#GFP-Atg8#ATG8 Endogenous Promoter#Starvation
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