Bar Plot from Scientific Research

GFP-Atg8 processing assay. cys 4Δ (Y1558) mutants expressing GFP-Atg8 from ATG8 endogenous promoter were grown and shifted to starvation medium as above. Samples were collected at the indicated times. GFP-Atg8 expression and GFP release are relative to the 6-h time point in the no sulfur condition. Data are mean of n = 3; n are independent experiments. Error bars indicate SEM. Statistical significance between the two conditions was determined by multiple two-sided t test comparisons using Holm-Sidak method. P values: *** p < 0.0001; ** p = 0.00095, 0.00017 and 0.00016; * p = 0.0072 and 0.0018; ns = 0.15, 0.43 and 0.25 (from left to right).

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