Bar Plot from Scientific Research

Simplified schematics of the sulfur-containing amino acid biosynthesis pathway indicating the steps interrupted in the met6Δ mht1Δ sam4Δ (upper panel) mutants. The sulfur compounds becoming depleted after transfer into the indicated starvation media are written in white on gray. Sulfur supplements are marked with a thick colored outline. The met6Δ mht1Δ sam4Δ strain (Y1533 or 1534) was grown in SF-medium supplemented with 0.1 mM Cys and Met, and shifted to SF-medium alone or containing 0.1 mM Cys and SAM. Samples were collected at the indicated times. Transcript levels (graphs) were measured by RT-qPCR. Fold induction is relative to t0. Data are mean of n = 2; n are independent experiments. Error bars indicate SEM.

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