GFP-Atg8 processing assay. Indicated strains expressing GFP-Atg8 from ATG8 endogenous promoter (Y1408, Y1611, Y1583 & Y1608) were grown and starved as above. Cells were collected at the indicated times and processed as described in Methods . Molecular weight are in kDa. GFP-Atg8 expression and GFP release are relative to the value in the WT at the 6-h time point. Data are mean SEM ( n = 3 independent experiments). Statistical significance compared with WT was determined as above. P -values, in each case from left to right: **** p < 0.0001; ** p = 0.0012, 0.0058 and 0.0016; ns = 0.99, 0.79, 0.96 and 0.99.
